Transgenic Animal Genotyping and Phenotyping Core

Center of Biomedical Research Excellence

Under the direction of Dr. Yumei Feng Earley, Ph.D., Core B aims to establish and operate a centralized facility that provides services, advice, training and technical support for evaluating transgenic animal genotyping and cardiovascular phenotyping research. Operations of a centralized facility is to provide an infrastructure for the success of cardiovascular research and facilitate the development of novel drugs for the treatment of cardiovascular diseases. Major Cardiovascular Phenotyping techniques offered by this core are continuous blood pressure, heart rate, ECG monitoring by radio telemetry system, in vivo calcium imaging & optogenetics in live free-moving mice, echocardiography, laser doppler blood flow recording.

Director and Staff

Dr. Yumei Feng                                                   Gerald Bustos Staff Research Associate      

Core Director                                                                           Genotyping Specialist    
Yumei Feng Earley, M.D., PhD. F.A.H.A.                          Gerald V. Bustos, MS
Email: yumeifeng@med.unr.edu                                     Staff Research Associate
Phone: (775) 784-4116                                                        Email: gbustos@med.unr.edu

List of genetic modified mice strains

Service and Facilities

• Breeding, genotyping, and management of colonies of genetically modified mice

A centralized mouse holding and breeding space is located in the facility of Animal Resource at University of Nevada, Reno. Genotyping of tail biopsy samples and data analysis will be performed. All data on mice, including date of birth, sex, cage IDs, genotype and other information, is uploaded to the mouse management system, mLIMS (https://bioinforx.com/lims2/product_mlims.php) and will be continuously available for retrieval by investigators. Many commonly used mouse strains at a wide range of ages are maintained in the facility. New strains or tissue-specific targeting can be requested by investigators and generated at the Core on an as-needed basis.

• Radio telemetry blood pressure, heart rate, ECG monitoring system

Telemetry recording suite is located in the facility of Animal Resource at University of Nevada, Reno. Two telemetry systems (Data Science International, Inc.) with the capacity to simultaneously monitor and record hemodynamic parameters (systolic, diastolic, and mean blood pressure; heart rate, ECG, and locomotor activity) in up to 48 conscious, unrestrained mice. The linchpin of telemetry recording in mice is a computer-controlled receiving plate that can be positioned beneath individual cages housing mice that have been surgically implanted with radiotelemetric transmitters.

• Vevo 2100 cardiovascular ultrasound system

This service is based on a Vevo 2100 cardiovascular ultrasound system (FUJIFILM VisualSonics, Toronto, ON, CA). The Vevo 2100 is among the most popular ultrasound systems on the market today. Its popularity stems from its outstanding image quality and long-term history as a solid, reliable instrument. In particular, it is well known for its outstanding image quality in all modalities-cardiac, vascular and general radiology-and high frame rates. It also offers Color and Power Doppler Modes for blood flow quantification and M-Mode single-line acquisition for high-temporal resolution in left ventricular functional analysis, and comes with nonlinear contrast imaging and VevoStrain analysis software for high-resolution image analysis of chamber dimensions, wall thicknesses, and ventricular function in lightly sedated mice or rats.

• Laser Doppler blood flow recording system

A laser Doppler flowmetry system (Periflux System 5000; Perimed) is set up for assessment of vascular function. The system consists of two main units, each equipped with four function units. The modular design enables simultaneous laser Doppler monitoring of blood flow and perfusion of the vasculature. Combined with dedicated software provided by Perimed, the system offers a dedicated analysis tool for assessing microcirculatory function.

• In vivo calcium imaging & optogenetics system
In development (coming soon...)