Non-Variola Orthopoxvirus (Vaccinia, monkeypox) DNA by real-time PCR Assay

NSPHL Clinical Analysis: Non FDA Approved

Non-Variola Orthopoxvirus (Vaccinia, monkeypox) DNA by real-time PCR Assay
Method Real Time Polymerase Chain Reaction (q-PCR)
Amplification of Non-Variola Orthopoxvirus DNA
CPT Code
Test Scope This assay to serve as an aid in determining whether Vaccinia is the causative agent of a vaccination adverse event in smallpox vaccines or their contacts.
Preferred Specimen
  • Dried vesicular fluid on a slide (touch prep)
  • Fresh biopsy
  • Skin or curst from roof of vesicle
  • Dry or wet swab of lesion
Required Specimen Volume 2-4 swabs or scab samples from lesions
Specimen Container Screw-cap 15 mL polypropylene tube
Specimen Identification Label container with patient’s first name, last name and DOB include date and time of collection and sample source.
Special Instructions Swabs or scabs, store refrigerated at 2-8°C or frozen (less than or equal to -20°C)
Shipping Requirements NSPHL can accept swabs stored in viral transport media (VTM) only and crusts for characterization testing. Swabs in VTM and/or crusts must be received by NSPHL within 7 days of collection. We recommend refrigerating (2- 8°C) or freezing (≤20°C) all specimens within an hour after collection. Whenever possible, ship specimens on dry ice. Specimens received outside of acceptable temperature ranges will be rejected.
Sample Rejection Criteria
  • Incomplete specimen labeling / documentation
  • Mislabeled/Unlabeled Specimen
  • Inappropriate Transport Media (Frozen or Room Temperature)
  • Specimens in transport medium
Turn-around-time 24-48 hours, Monday through Friday