The brain requires a tremendous amount of energy to fuel its normal moment-to-moment computation without having its own energy reserves. Thus, it needs a continuous blood supply that can be dynamically regulated to meet the ever-changing metabolic demands. Insufficient supply of nutrients will lead to cell death and consequent sensory, motor, and cognitive deficits as seen in conditions like stroke and dementia. The cellular communication between different members of the neurovascular unit plays a critical role in regulating blood flow control and consequently the functionality of neural cells in the brain. Our laboratory uses two-photon fluorescence imaging, electrophysiology, chemogenetics, optogenetics and complementary pressure myography to gain insights into the interaction between members of the neurovascular unit in health and disease in hope of uncovering potential targets for diagnosis and treatment.