Publications
Yan Lab
Selected publications
2015
- Yuan S, McSinggin H, Zheng H, Yan W. . A Testis-Specific Gene, Ubqlnl, Is Dispensable for Mouse Embryonic Development and Spermatogenesis. Molecular Reproduction and Development 82: 408-409. DOI 10.1002/mrd.22504.
- Oliver DK, Yuan S, Yan W. . Cas9/CRISPR-mediated genome editing results in pervasive genotypic mosaicism in founder mice derived from pronuclear injections. PLoS ONE 0(6): e0129457. doi:10.1371/journal.pone.0129457.
- Song HW, Bettegowda A, Oliver D, Yan W, Phan MH, de Rooij DG, Corbett M, Wilkinson MF. . shRNA Off-Target Effects In Vivo: Impaired Endogenous siRNA Expression and Spermatogenic Defects. PLoS ONE In Press.
- Yuan S, Oliver DK, Schuster A, Zheng H, Yan W. . Breeding scheme and maternal small RNAs affect the efficiency of transgenerational inheritance of a paramutation in mice. Scientific Reports In Press.
- Yuan S, Qin W, Riordan CR, McSwiggin, H, Zheng H, Yan W. . Ubqln3, a testis-specific gene, is dispensable for embryonic development and spermatogenesis in mice. Molecular Reproduction and Development In Press.
- Ortogero N, Hennig GW, Luong D, Yan W. . Computer-assisted annotation of small RNA transcriptomes. Methods in Molecular Biology 2015;1218:353-64. doi:10.1007/978-1-4939-1538-5_22. PubMed PMID: 25319663.
- Schuster A, Hennig GW, Ortogero N, Luong D, Yan W. . In Silico Identification of Novel Endo-siRNAs. Methods in Molecular Biology. 2015;1218:341-51. doi:10.1007/978-1-4939-1538-5_21. PubMed PMID: 25319662.
- Yuan S, Tang C, Zhang Y, Wu J, Bao J, Zheng H, Xu C, Yan W. . mir-34b/c and mir-449a/b/c are required for spermatogenesis, but not for the first cleavage division in mice. Biology Open Advance Online Article January 23, 2015, doi:10.1242/bio.201410959.
- Yuan S, Stratton CJ, Bao J, Zheng H, Bhetwal BP, Yanagimachi, R, Yan W. . Spata6 is required for normal assembly of the sperm connecting piece and tight head-tail conjunction. Proceedings of the National Academy of Sciences USA Published online before print January 20, 2015, doi:10.1073/pnas.1424648112 PNAS January 20, 2015.
2014
- Bao J, Tang C, Li J, Zhang Y, Bhetwal BP, Zheng H, Yan W. . RAN-Binding Protein 9 is Involved in Alternative Splicing and is Critical for Male Germ Cell Development and Male Fertility. PLoS Genet 10(12): e1004825. doi:10.1371/journal.pgen.1004825.
- Bao J, Tang C, Porse BT, Yan W. . UPF2, a nonsense-mediated mRNA decay factor, is required for prepubertal Sertoli cell development and male fertility by ensuring transcriptomic fidelity. Development IN PRESS.
- Ortogero N, Hennig GW, Luong D, Yan W. . Computer-assisted annotation of small RNA transcriptomes. Methods in Molecular Biology. 2015;1218:353-64. doi:10.1007/978-1-4939-1538-5_22. PubMed PMID: 25319663.
- Ortogero N, Schuster AS, Oliver DK, Riordan CR, Hong AS, Hennig GW, Luong D, Bao J, Bhetwal BP, Ro S, McCarrey JR, Yan W. . A Novel Class of Somatic Small RNAs Similar to Germ Cell Pachytene PIWI-interacting Small RNAs. Journal of Biological Chemistry. 2014 Oct15. pii: jbc.M114.613232. [ePub ahead of print] PubMed PMID: 25320077. doi: 10.1074.
- Yan W. . Potential roles of noncoding RNAs in environmental epigenetic transgenerational inheritance. Molecular and Cellular Endocrinology, Available online 16 September 2014, DOI: 10.1016/j.mce.2014.09.008.
- Yuan S, Ortogero N, Wu Q, Zheng H, Yan W. . Murine Follicular Development Requires Oocyte DICER, but Not DROSHA. Biology of Reproduction Published online before print July 2, 2014, doi: 10.1095/biolreprod.114.119370.
- Wu J, Bao J, Kim M, Yuan S, Tang C, Zheng H, Mastick G, Xu C, Yan W. . Two miRNA clusters, miR-34b/c and miR-449, are essential for normal brain development, motile ciliogenesis, and spermatogenesis. Proceedings of the National Academy of Sciences, 111(28), E2851-E2857. Published online before print June 30, 2014, doi: 10.1073/pnas.1407777111.
- Bao J, Zhang Y, Schuster A, Ortogero N, Nilsson EE, Skinner MK, Yan W. . Conditional inactivation of Miwi2 reveals that MIWI2 is only essential for prospermatogonial development in mice. Cell Death and Differentiation (24 January 2014) doi:10.1038/cdd.2014.
2013
- Bao J, Ma HY, Schuster A, Lin YM, Yan W. . Bao J, Ma HY, Schuster A, Lin YM, Yan W. Genesis Published online on March 12, 2013, DOI: 10.1002/dvg.22389.
- Bao J, Wu J, Schuster AS, Hennig GW, Yan W. . Expression Profiling Reveals Developmentally Regulated lncRNA Repertoire in the Mouse Male Germline. Biology of Reproduction Published online before print September 18, 2013, doi: 10.1095/biolreprod.113.113308.
- Yuan S, Zheng H, Zheng Z, Yan W. . Proteomic analyses reveal a role of cytoplasmic droplets as an energy source during epididymal sperm maturation. PLoS ONE 8(10): e77466. doi:10.1371/journal.pone.0077466.
- Bao J, Yuan S, Maestas A, Bhetwal B, Schuster A, Yan W. . Stk31 is dispensable for embryonic development and spermatogenesis in mice. Molecular Reproduction and Development. Volume 80, Issue 10, page 786, October 2013.
- Xu H, Yuan S, Zheng Z, Yan W. . The cytoplasmic droplet may be indicative of sperm motility and normal spermiogenesis. Asian Journal of Andrology June 17, 2013; doi:10.1038/aja.2013.69.
- Ro S, Ma HY, Park C, Ortogero N, Song R, Hennig GW, Zheng H, Lin YM, Moro L, Hsieh JT, Yan W. . The Mitochondrial Genome Encodes Abundant Small Noncoding RNAs. Cell Research Published online on 12 March 2013, doi:10.1038/cr.2013.37.
2012
- Idler, KR, Hennig, GW, Yan W. . Bioinformatic Identification of Novel Elements Potentially Involved in mRNA Fate Control during Spermatogenesis. Biology of Reproduction 87 (6) 138:1-10. Published ahead of print October 10, 2012, doi: 10.1095/biolreprod.112.102434.
- Ortogero N, Hennig GW, Langille C, Ro S, McCarrey JR, Yan W. . Computer-Assisted Annotation of the Sertoli Cell Small RNA Transcriptome. Biology of Reproduction 88 (1) 3:1-10. Published ahead of print November 7, 2012, doi:10.1095/biolreprod.112.102269.
- Bao J, Yan W. . Male germline control of transposable elements. Biology of Reproduction 86 (5) 162, 1-14.
- Bao J, Li D, Wang L, Wu J, Hu Y, Wang Z, Chen Y, Cao X, Jiang C, Yan W*, Xu C. . microRNA-449 and microRNA-34b/c function redundantly in murine testes by targeting the E2F transcription factor-Retinoblastoma protein (E2F-pRb) pathway. Journal of Biological Chemistry 287(26): 21686-21698. (*co-corresponding author).
- Wu Q, Song R, Ortogero N, Zheng H, Evanoff R, Small CL, Griswold MD, Namekawa SH, Royo H, Turner JM, Yan W. . The RNase III Enzyme DROSHA Is Essential for microRNA Production and Spermatogenesis. Journal of Biological Chemistry 287(30): 25173-25190.
2011
- Idler RK, Yan W. . Control of messenger RNA fate by RNA binding proteins: an emphasis on mammalian spermatogenesis. Journal of Andrology 33: 309-337.
- Song R, Hennig G, Wu Q, Jose C, Zheng H, Yan W. . Male germ cells express abundant endogenous siRNAs. Proceedings of the National Academy of Sciences USA. 108:13159-64.
- Park C, Yan W, Ward SM, Hwang SJ, Wu Q, Hatton W, Park J, Sanders KM, Ro S. . MicroRNAs dynamically remodel gastrointestinal smooth muscle cells. PLoS ONE 6(4): e18628.
- Bao J, Zhang J, Song R, Zheng H, Yan W. . Ran-binding protein 17 is preferentially expressed in the testis and is associated with the XY body and manchette during spermatogenesis. Molecular and Cellular Endocrinology 333:134-142.
- Park C, Hennig GW, Sanders KM, Cho JH, Hatton WJ, Redelman D, Park JK, Ward SM, Miano JM, Yan W, Ro S. . SRF-dependent microRNAs regulate gastrointestinal smooth muscle cell phenotypes. Gastroenterology 141(1): 164-175.
- Bao J, Wang L, Lei J, Hu Y, Liu Y, Shen H, Yan W, Xu C. . STK31(TDRD8) is dynamically regulated throughout mouse spermatogenesis and interacts with MIWI protein. Histochemistry and Cell Biology 137(3): 377-89.
2010
- Ro S, Park C, Jin J, Zheng H, Blair P, Redelman D, Ward SM, Yan W, Sanders KM. . A model to study the phenotypic changes of interstitial cells of Cajal (ICC) in gastrointestinal diseases. Gastroenterology 138(3): 1068-78.e1-2.
- Ro S, Yan W. . Detection and quantitative analysis of small RNAs by PCR. Methods in Molecular Biology 629:295-305.
- Song R, Ro S, Yan W. . In situ hybridization detection of microRNAs. Methods in Molecular Biology 629:287-94.
- Ro S, Yan W. . Small RNA cloning. Methods in Molecular Biology 629:273-85.
- Bao J, Zhang J, Zheng H, Yan W. . Ubiquilin1is localized to the junction of Sertoli cell and developing spermatids. Molecular and Cellular Endocrinology 327:89-97.
- Yan W, Slaymaker S, Si Y, Jin J, Zheng H, Aslanian A, Saunders L, Verdin E, Charo IF. . Zmynd15 encodes a transcription repressor essential for late spermiogenesis and male fertility. Journal of Biological Chemistry 285:31418-31426.
2009
- Yan W, McCarrey JR. . Noise amidst the silence: sex chromosome inactivation in the male. Epigenetics 4(7):452-456.
- Zhu MH, Kim TW, Ro S, Yan W, Ward SM, Koh SD, Sanders KM. . A Ca(2+)-activated Cl(-) conductance in interstitial cells of Cajal linked to slow wave currents and pacemaker activity. Journal of Physiology 587:4905-4918.
- Ma L, Buchold GM, Greenbaum MP, Roy A, Burns KH, Zhu H, Han DY, Harris RA, Coarfa C, Gunaratne PH, Yan W, Matzuk MM. . GASZ is Essential for Male Meiosis and Suppression of Retrotransposon Synthesis in the Male Germline. PLoS Genetics 5(9): e1000635. Published online 2009 September 4. doi: 10.1371/journal.pgen.1000635.
- Yan W. . Male Infertility caused by spermiogenic defects: Lessons from gene knockouts. Molecular and Cellular Endocrinology 306:24-32. doi:10.1016/j.mce.2009.03.003.
- Papaioannou MD, Pitetti JL, Ro S, Park C, Aubry F, Schaad O, Vejnar CE, Descombes P, Zdobnov EM, McManus MT, Guillou F, Harfe BD, Yan W, Jégou B, Nef S. . Sertoli cell Dicer is essential for spermatogenesis in mice. Developmental Biology 326:250-259.
- Song R, Ro S, Michaels JD, Park, C, McCarrey JR, Yan W. . X-linked microRNAs escape meiotic sex chromosome inactivation. Nature Genetics 41:488-493.
2008
- Yan W*, Morozumi K*, Zhang J, Ro S, Park C, Yanagimachi R. . Birth of mice after intracytoplasmic injection of single purified sperm nuclei and detection of messenger RNAs and microRNAs in the sperm nuclei. Biology of Reproduction 78:896-902 (*corresponding author).
2007
- Jin J, Jin N, Zheng H, Ro S, Tafolla D, Sanders KM, Yan W. . Catsper3 andCatsper4 are Essential for Sperm Hyperactivated Motility and Male Fertility. Biology of Reproduction 77:37-44.
- Ro S, Song R, Park C, Zheng H, Sanders KM, Yan W. . Cloning and expression profiling of small RNAs expressed in the mouse ovary. RNA Published online before print October 19, 2007, DOI: 10.1261/rna.754207.
- Ro S, Park C, Young D, Sanders KM, McCarrey JR, Yan W. . Cloning and expression profiling of testis-expressed microRNAs. Developmental Biology 311: 604-614.
- Ro S, Park C, Song R, Nguyen D, Jin J, Sanders KM, McCarrey JR, Yan W. . Cloning and expression profiling of testis-expressed piRNA-like RNAs. RNA Published August 13, 2007, 10.1261/rna.640307.
- Zheng Z, Zheng H, Yan W. . Fank1 is a testis-specific gene encoding a nuclear protein exclusively expressed during the transition from the meiotic to the haploid phase of spermatogenesis. Gene expression patterns 7:777-783.
- Zheng H, Stratton CJ, Morozumi K, Jin J, Yanagimachi R, Yan W. . Lack of Spem1 causes aberrant cytoplasm removal, sperm deformation and male infertility. Proceedings of the National Academy of Sciences USA 104:6852-6857.
- Lin YN, Roy A, Yan W, Burns KH, Matzuk MM. . Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis. Molecular and Cellular Biology doi:10.1128/MCB.01029-07, published online ahead of print on 30 July 2007.
- Ro S, Park C, Young D, Sanders KM, Yan W. . Tissue-dependent paired expression of miRNAs. Nucleic Acids Research Published online on August 28, 2007, Doi:10.1093/nar/gkm641.
2006
- Ro S, Park C, Jin J, Sanders KM, Yan W. . A PCR-based method for detection and quantification of small RNAs. Biochemical and Biophysical Research Communications 351:756-763.
- Wang SH, Zheng HL, Esaki Y, Kelly F, Yan W. . Cullin3 is a KLHL10-interacting protein preferentially expressed during late spermiogenesis. Biology of Reproduction 74:102-108.
- Yatsenko AN, Roy A, Chen R, Ma L, Murthy LJ, Yan W, Lamb DJ, Matzuk MM. . Non-invasive genetic diagnosis of male infertility using spermatozoal RNA: KLHL10 mutations in oligozoospermic patients impair homodimerization. Human Molecular Genetics 15:3411-3419.
- Greenbaum MP, Yan W*, Wu MH, Lin YN, Agno JE, Sharma M, Braun RE, Rajkovic A, Matzuk MM. . TEX14 is essential for intercellular bridges and fertility in male mice. Proceedings of the National Academy of Sciences USA103(13):4982-7. (*co-first authors).
2005
- Jin JL, O’Doherty AM, Wang SH, Zheng HL, Sanders KM, Yan W. . Catsper3and Catsper4 encode two cation channel-like proteins exclusively expressed in the testis. Biology of Reproduction 73:1235-1242.
- Yan W, Ma L, Stein P, Pangas SA, Burns KH, Bai, YC, Schultz, RM, Matzuk, MM. . Mice deficient in oocyte-specific oligoadenylate synthetase-like protein OAS1D display reduced fertility. Molecular and Cellular Biology 25:4615-4624.
- Huang JX, Yan W, Song ZX, Qian RY, Chen P, Salminen E, Toppari J. . Relationship between proliferative activity of cancer cells and clinic pathological factors in patients with esophageal squamous cell carcinoma. World Journal of Gastroenterology 11:2956-2959.
2004
- Yan W, Ma L, Burns, KH, Matzuk MM. . Haploinsufficiency of KLHL10 causes male infertility. Proceedings of the National Academy of Sciences USA101:7793-7798.
- Yan W, Ma L, Zilinski CA, Matzuk MM. . Identification and characterization of evolutionarily conserved pufferfish, zebrafish, and frog orthologs of GASZ. Biology of Reproduction 70:1619-1625.
- Pangas SA, Yan W., Matzuk MM, Rajkovic A. . Restricted germ cell expression of a gene encoding a novel mammalian HORMA domain containing protein. Gene Expression Patterns 5: 257-263.
- Roy A, Yan W, Burns KH, Matzuk MM. . Tektin3 encodes an evolutionarily conserved putative testicular microtubules-related protein expressed preferentially in male germ cells. Molecular Reproduction and Development 67:295-302.
2003
- Yan W, Assadi AH, Wynshaw-Boris A, Eichele G, Matzuk MM, Clark GD. . A novel role for platelet-activating factor acetylhydrolase 1b in mouse spermatogenesis. Proceedings of the National Academy of Sciences USA100:7189-7194.
- Yan W, Santti H, Jänne OA, Palvimo JJ, Toppari J. . Expression of the E3 SUMO-1 ligases PIASx and PIAS1 during spermatogenesis in the rat. Gene Expression Patterns 3:301-308.
- Yan W, Burns KH, Matzuk MM. . Genetic engineering to study testicular tumorigenesis (Review). APMIS 111: 174-183.
- Yan W, Ma L, Burns KH, Matzuk MM. . Identification of a novel spermatid-specific linker histone H1 involved in chromatin remodeling during mammalian spermiogenesis. Proceedings of the National Academy of Sciences USA100:10546-10551.
- Yan W, Huang JX, Lax AS, Pelliniemi L, Salminen E, Poutanen M, Toppari J. . Overexpression of Bcl-w in the testis disrupts spermatogenesis: revelation of a role of BCL-W in male germ cell cycle control. Molecular Endocrinology 17:1868-1879.
- Suzumori N, Burns KH, Yan W, Matzuk MM. . RFLP-4 interacts with proteins of the ubiquitin-proteosome degradation pathway in oocytes. Proceedings of the National Academy of Sciences USA 100:550-555.
- Wu MH, Rajkovic A, Burns KH, Yan W, Lin YN, Matzuk MM. . Sequence and expression of testis-expressed gene 14 (Tex 14): a gene encoding a protein kinase expressed exclusively during spermatogenesis. Gene Expression Patterns 3:231-236.
- Toppari J, Suominen JS, Yan W. . The role of retinoblastoma protein family in the control of germ cell proliferation, differentiation, and survival (Review). APMIS 111:245-251.
2002
- Yan W, Hirvonen SJ, Palvimo J, Toppari J, Janne OA. . Expression of the Nuclear RING Finger Protein SNURF/RNF4 during Rat Testis Development Suggests a Role in Spermatid Maturation. Mechanisms of Development 118: 247-253.
- Yan W, Rajkovic A, Viveiros MM, Burns KH, Eppig JJ, Matzuk MM. . Identification of Gasz, an evolutionarily conserved gene expressed exclusively in germ cells and encoding a protein with four ankyrin repeats, a sterile-alpha motif, and a basic leucine zipper. Molecular Endocrinology 16: 1168-1184.
- Yan W, Burns KH, Ma L, Matzuk MM. . Identification of Zfp393, a germ cell-specific gene encoding a novel zinc finger protein. Mechanisms of Development 118: 233-239.
- Rajkovic A, Yan C, Yan W, Klysik M, Matzuk M. . Obox, a family of homeobox genes preferentially expressed in germ cells. Genomics 79: 711-717.
2001
- Li X, Nokkala E, Yan W, Streng T, Saarinen N, Wärri A, Huhtaniemi I, Santti R, Mäkelä S, Poutanen M. . Altered structure and function of reproductive organs in transgenic male mice overexpressing human aromatase and maintaining elevated serum estradiol and low testosterone concentrations. Endocrinology 142:2435–2442.
- Yan W, Kero J, Suominen J, Toppari J. . Differential expression and regulation of retinoblastoma protein (pRb) family members during testicular development and spermatogenesis: a model for the control of germ cell proliferation, differentiation and apoptosis in vivo. Oncogene 20: 1343–1356.
- Blaeser F, Toppari J, Heikinheimo M, Yan W, Wallace M, Ho N, Chatila T. . Normal Spermatogenesis in CaMKIV/Gr-Deficient Mice. American Journal of Physiology, Endocrinology, and Metabolism 281: E931-E937.
- Suominen J, Yan W, Toppari J, Kaipia A. . The expression and regulation of Bcl-2-related ovarian killer (Bok) mRNA in the developing and adult rat testis. European Journal of Endocrinology 145:771-778.
2000
- Yan W, Samson M, Jégou B, Toppari J. . Bcl-w forms complexes with Bax and Bak and elevated ratios of Bax/Bcl-w and Bak/Bcl-w correspond to spermatogonial and spermatocyte apoptosis in the testis. Molecular Endocrinology 14:682-699.
- Zheng H, Yan W, Toppari J, Härkönen P. . Improved non-radioactive RT-PCR method for relative quantification of mRNA. BioTechniques 28:832-834.
- Yan W, Suominen J, Samson M, Jégou B, Toppari J. . Involvement of Bcl-2 family proteins in apoptosis during testicular development and pro-survival effect of stem cell factor on germ cells in vitro
- Yan W, Kero J, Huhtaniemi I, Toppari J. . Stem cell factor functions as a survival factor for mature Leydig cells and a growth factor for precursor Leydig cells after ethylene dimethane sulfonate treatment: implication of the role of stem cell factor/c-kit interaction in Leydig cell development. Developmental Biology 227:169-182.
- Yan W, Suominen J, Toppari J. . Stem cell factor protects germ cells from apoptosis in vitro. Journal of Cell Science 113:161-168.
1999
- Moilanen AM, Karvonen U, Poukka H, Yan W, Toppari J, Jänne OA, Palvimo J. . A testis-specific androgen receptor coregulator that belongs to a novel family of nuclear proteins. Journal of Biological Chemistry 274:3700-3704.
- Nata M, Kimura T, Hashiyada M, He P, Yan W, Li X, Funayama M, Sagisaka K. . Allele frequencies of eight STRs in Japanese and Chinese. International Journal of Legal Medicine 112:396-399.
- Schultz R, Yan W, Toppari J, Völkl A, Gustafsson J, Huikko MP. . Expression of peroxisome proliferator-activated receptor α mRNA and protein in human and rat testis. Endocrinology 140:2968-2975.
- Hakovirta H*, Yan W*, Kaleva M, Zhang F, Vänttinen K, Morris PL, Söder O, Parvinen M, Toppari J. . Function of stem cell factor as a survival factor of spermatogonia and localization of messenger ribonucleic acid in the rat seminiferous epithelium. Endocrinology 140:1492-1498 (*co-first authors).
- Yan W, Linderborg J, Suominen J, Toppari J. . Stage-specific regulation of stem cell factor (SCF) gene expression in the rat seminiferous epithelium. Endocrinology 140:1499-1504.
- Tena-Sempere M, Kero J, Rannikko A, Yan W, Huhtaniemi I. . The pattern of inhibin/activin α and βB subunit mRNA expression in rat testis after selective Leydig cell destruction by ethylene dimethane sulfonate
1997
- He P, Nata M, Kanetake J, Ji G, Yan W, Li X, Sagisaka K. . Polymorphism of short tandem repeat (STR) loci PLA2, D3S2459, D8S315 (kw38), CYP19, D3S1359, FGA, and D8S1132 in the Japanese and Chinese populations. Tohoku Journal of Experimental Medicine 183:251-262.
- Yan W, West A, Toppari J, Lähdetie J. . Stage-specific expression and phosphorylation of retinoblastoma protein (pRb) in the rat seminiferous epithelium. Molecular and Cellular Endocrinology 132:137-148.
1995
- Sun YX, Zhao GC, Yan W. . Age estimation on the female sternum by quantification theory and stepwise regression analysis. Forensic Science International 74:57-62.
1993
- Jiang XH, Yan W. . Parentage determination of placental tissues through DNA fingerprints. Annals of Academy of Medicine 22:8-10.