| Method |
Enzyme Linked Immunosorbent Assay (ELISA) |
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| CPT Code |
86790 |
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| Preferred Specimen |
Serum |
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| Required Specimen Volume |
1-3 ml serum, Minimum 1 mL |
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| Specimen Container |
Red Top or SST Tube, 7-10 mL, separated serum is preferred |
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| Specimen Identification |
Label tube with patient’s first and last name, DOB and date/time of collection. Note ACUTE or CONVALESCENT sample type.
- ACUTE: 3-10 days after onset of symptoms
- CONVALESCENT: 2-3 weeks after acute specimen
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| Special Instructions |
- Centrifuge and remove serum from clot as soon as possible before storing. Transfer separated serum into a labeled plastic transport tube.
- Sample may be stored up to 48 hours at 2-8° C
- If need to store longer than 48 hours, serum should be frozen at -20° C, CSF should be refrigerated
- Specimens with precipitate should be clarified by centrifugation
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| Shipping Requirements |
Store and ship at refrigerated temperature 2-8° C if within 48 hours. Store and ship serum frozen if > 48 hours after collection. |
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| Sample Rejection Criteria |
- Heat-inactivated serum
- Gross Hemolysis
- Lipemia
- Mislabeled/Unlabeled specimen
- Gross Bacterial Contamination
- Quantity not sufficient for testing (QNS)
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| Turn-around-time |
7 Days |
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