| Method |
Enzyme Immunoassay (EIA) |
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| CPT Code |
86794 |
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| Preferred Specimen |
Serum |
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| Other Acceptable Specimens |
Acute and convalescent human serum collected in a serum separator tube; cerebral spinal fluid (CSF) only with a matched serum |
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| Required Specimen Volume |
Minimum of 0.25 ml of serum |
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| Minimum Specimen Volume |
Minimum of 0.25 ml of serum |
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| Specimen Container |
SST Tube; sterile container for CSF |
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| Specimen Identification |
Label tube with patient's first and last name, DOB and date/time of collection. |
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| Special Instructions |
- Sample may be stored up to 7 days refrigerated at 2-8° C.
- Centrifuge and remove serum from clot as soon as possible and before storing. Transfer separated serum or plasma to a labeled plastic transport tube.
- If need to store longer than 7 days, serum or plasma may be frozen at -20° C or colder.
- Specimens with precipitate should be clarified by centrifugation.
- Do not use heat-inactivated specimens.
- Serum collection tubes should not be used with heparinized patients. Use plasma collection tubes.
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| Shipping Requirements |
Store and ship at refrigerated temperature 2-8° C. |
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| Sample Rejection Criteria |
- Grossly Lipemic specimen
- Gross Hemolysis
- Improper specimen transport or storage
- Mislabeled/Unlabeled specimen
- Heat-inactivated specimen
- Specimen quantity not sufficient for testing (QNS)
- Specimens containing sodium azide.
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| Reference Interval |
Negative |
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| Turn-around-time |
72 hours |
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