Arbovirus Detection by Real Time PCR (Dengue, Chikungunya, Zika)

Influenza Screen and Sub-Typing Real-time (TaqMan®) RT-PCR
DataValue
Method Real Time Reverse Transcription Polymerase Chain Reaction (q-PCR);  Amplification of Dengue, Chikungunya and Zika virus RNA
CPT Code 87798
Test Scope Qualitative detection of Dengue Virus, Chikungunya Virus and Zika Virus
Preferred Specimen

For Dengue and Chikungunya:  serum or cerebral spinal fluid (CSF) collected alongside a matched serum specimen

For Zika:  urine or amniotic fluid
Specimen Container serum collection tube; urine collection container; appropriately sealed plastic tube (CSF)
Specimen Identification Label tube with patient’s first and last name, DOB and date/time of collection.
Special Instructions
  • Indicate site of collection or source on requisition.
  • Include the date the sample was collected, date of onset of illness, a brief clinical description or the provisional diagnosis as well as any other pertinent clinical information on the requisition.
  • Onset date, specimen collected date, travel dates, and travel location(s) should be included
Shipping Requirements Specimen should be kept refrigerated at 4°C and shipped cold on gel ice-packs. Frozen specimens (less than or equal to -20⁰C) should be shipped on dry ice.
Sample Rejection Criteria
  • Swabs with calcium alginate, cotton tips or wooden shafts.
  • Swab not in viral transport media.
  • Specimen quantity not sufficient (QNS)
  • Specimen not refrigerated
  • Mislabeled/Unlabeled specimen
Turn-around-time 48 hours