Clostridium botulinum (Botulism) Toxins A, B, E and F by ELISA assay

Data Value
Method In vitro detection of Botulinum Toxins A, B, E and F by ELISA (This test is referred to the Utah State Public Heatlh Laboratory)
CPT Code N/A
Testing Scope Not cleared for human clinical specimens and must be confirmed by mouse bioassay.
Preferred Specimen
  • Culture supernatants
  • Suspect food or drink in original container
  • Environmental samples of soil, water or swab
Required Specimen Volume Sufficient sample must be submitted to confirm positives by Mouse Bioassay
  • 10 mL culture supernatant
  • 1-2 grams suspect food in original container
  • 3-4 Environmental Swabs from each potential site
Specimen Container Leak proof containers
Special Instructions Notify local Health Authority AND the Nevada State Epidemiologist ((775) 684-5900) with any suspected botulism case.
Shipping Requirements
  • Culture or Food: store and ship refrigerated temperature
  • Environmental Swabs: store and ship room temperature
Sample Rejection Criteria
  • Specimens not kept refrigerated. DO NOT freeze
  • QNS sample to run ELISA and mouse bioassay
Turn-around-time 1-2 weeks (This test is referred to the Utah State Public Heatlh Laboratory)
Testing Location Utah Division of Epidemiology and Lab Services
Salt Lake City, UT 84113-1105