Ebola Virus Real-time PCR (Qualitative)

E. coli O157:H7 Confirmation
Data	Value
Method	Real-time polymerase chain reaction (RT-PCR) Amplification of Ebola DNA.
CPT Code	A984
Preferred Specimen	4 mL whole blood.
Other Acceptable Specimens	Serum, Plasma, or Urine.
Specimen Container	Purple-Top tube for whole blood. For other collections see guidance below.
Specimen Identification	Label specimen with patient’s first and last name, DOB and date/time of collection.
Special Instructions	Prior to collection please read the PDF resource per CDC: Collection, Transport and Submission of Specimens for Ebola Virus Testing. Indicate specimen source on requisition.
Shipping Requirements	Contact NSHL prior to shipment. Specimen should be kept at 4°C (refrigerated) and transported to the laboratory within 24 hours of collection. Ship with cold-pack.
Sample Rejection Criteria	Failure to contact NSHL before shipment Improper specimen transport Mislabeled specimen Unlabeled specimen Wood shaft swabs
Turn-around-time	24-48 Hours (M-F)