Measles Virus RNA Real-time (TaqMan®) RT-PCR
| Data | Value |
|---|---|
| Method | Real-Time RT-PCR, Real Time Reverse Transcription Polymerase Chain Reaction (q-PCR) Amplification of Measles Virus (SH) RNA. |
| CPT Code | 87798 |
| Test Scope | |
| Collection Instructions |
Detection of measles RNA and measles virus isolation are most successful when samples are collected on the first day of rash through the 3 days following onset of rash. However, virus may still be recovered by cell culture through day 10 following rash onset. Detection of measles RNA by RT-PCR may be successful as late as 10-14 days post rash onset. Throat, Nasopharyngeal (preferred), or nasal swabs. Synthetic swabs are recommended. Swab should be transferred to 1-3ml of viral transport medium (do not allow to dry out). Indicate specimen source on requisition. |
| Other Acceptable Specimens | |
| Required Specimen Volume | |
| Specimen Container | Viral Transport Media. |
| Specimen Identification | Label specimen with patient's first and last name, DOB, and date/time of collection. |
| Shipping Requirements | Specimen should be kept at 4°C (refrigerated) and transported to the laboratory within 24 hours of collection. Ship with cold-pack. If longer storage is required, the specimen should be frozen at -70 °C or on dry ice. |
| Sample Rejection Criteria |
|
| Turn-around-time | 48-72 Hours (M-F) |