Monkeypox Detection by PCR
Non-Variola Orthopoxvirus (Vaccinia, monkeypox) DNA by real-time PCR Assay
NSPHL Clinical Analysis: Non FDA Approv
| Data | Value |
|---|---|
| Method | Real Time Polymerase Chain Reaction (q-PCR) Amplification of Non-Variola Orthopoxvirus DNA |
| CPT Code | |
| Test Scope | This assay to serve as an aid in determining whether Vaccinia is the causative agent of a vaccination adverse event in smallpox vaccines or their contacts. |
| Preferred Specimen |
|
| Required Specimen Volume | 2-4 swabs or scab samples from lesions |
| Specimen Container | Screw-cap 15 mL polypropylene tube |
| Specimen Identification | Label container with patient’s first name, last name and DOB include date and time of collection and sample source. |
| Special Instructions | Swabs or scabs, store refrigerated at 2-8°C or frozen (less than or equal to -20°C) |
| Shipping Requirements | NSPHL can accept swabs stored in viral transport media (VTM) only and crusts for characterization testing. Swabs in VTM and/or crusts must be received by NSPHL within 7 days of collection. We recommend refrigerating (2- 8°C) or freezing (≤20°C) all specimens within an hour after collection. Whenever possible, ship specimens on dry ice. Specimens received outside of acceptable temperature ranges will be rejected. |
| Sample Rejection Criteria |
|
| Turn-around-time | 24-48 hours, Monday through Friday |