NSPHL Service Request Form
| Data |
Value |
| Method |
Formalin Concentrate and Trichrome Stain |
| CPT Code |
87177, 8720 |
| Test Scope |
Includes concentration of material and examination of specimen for ova and parasite by conventional iodine/saline and Trichrome staining. This will not detect Cryptosporidium, Cyclospora or Microsporidium. |
| Preferred Specimen |
Stool |
| Other Acceptable Specimens |
Sputum or Urine |
| Required Specimen Volume |
5 mLs stool or sputum, 15 mLs Urine |
| Minimum Specimen Volume |
>2 mLs stool or sputum |
| Specimen Container |
- Stool or Sputum Sample: Two vial kit with 10% Formalin and PVA (Deposit stool sample in each container)
- Urine Sample: Use standard urine cup with screw cap and collect sample at mid-day.
|
| Specimen Identification |
Label container with patient's first and last name, DOB and date/time of collection. |
| Special Instructions |
- Stool specimens should be put into the two vials immediately after passage and thoroughly mixed with the solution up to the fill line.
- Multiple specimens should be collected on separate days. At least 3 specimens, one sample collected every 48 hours for a period of 5-6 days. Label and date each specimen as collected.
- Do not refrigerate the samples, store at room temperature
- Consult the microbiology department for handling of samples other than stool.
- Include any pertinent clinical and travel history on the test requisition.
|
| Shipping Requirements |
Store and ship at room temperature |
| Sample Rejection Criteria |
- Leaking Specimens
- Mislabeled/Unlabeled specimens
- Specimens delayed in transport
- Dry specimen
- Overfilled or mixed improperly
- Specimen containing substances such as bismuth, barium, magnesium, or mineral oil.
|
| Turn-around-time |
72 hours |