| Method |
Formalin Concentrate and Trichrome Stain |
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| CPT Code |
87177, 8720 |
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| Test Scope |
Includes concentration of material and examination of specimen for ova and parasite by conventional iodine/saline and Trichrome staining. This will not detect Cryptosporidium, Cyclospora or Microsporidium. |
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| Preferred Specimen |
Stool |
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| Other Acceptable Specimens |
Sputum or Urine |
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| Required Specimen Volume |
5 mLs stool or sputum, 15 mLs Urine |
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| Minimum Specimen Volume |
>2 mLs stool or sputum |
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| Specimen Container |
- Stool or Sputum Sample: Two vial kit with 10% Formalin and PVA (Deposit stool sample in each container)
- Urine Sample: Use standard urine cup with screw cap and collect sample at mid-day.
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| Specimen Identification |
Label container with patient’s first and last name, DOB and date/time of collection. |
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| Special Instructions |
- Stool specimens should be put into the two vials immediately after passage and thoroughly mixed with the solution up to the fill line.
- Multiple specimens should be collected on separate days. At least 3 specimens, one sample collected every 48 hours for a period of 5-6 days. Label and date each specimen as collected.
- DO NOT refrigerate the samples, store at room temperature
- Consult the microbiology department for handling of samples other than stool.
- Include any pertinent clinical and travel history on the test requisition.
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| Shipping Requirements |
Store and ship at room temperature |
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| Sample Rejection Criteria |
- Leaking Specimens
- Mislabeled/Unlabeled specimens
- Specimens delayed in transport
- Dry specimen
- Overfilled or mixed improperly
- Specimen containing substances such as bismuth, barium, magnesium, or mineral oil.
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| Turn-around-time |
72 hours |
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