Summary
Research
In my research laboratory, we focus on investigating smooth muscle physiology and motor neurotransmission from peripheral nerves, specifically in the gastrointestinal (GI) and bladder muscles. Our primary emphasis is on understanding the role of interstitial pacemaker cells in generating and regulating motility. To achieve this, we delve into the mechanisms of calcium signaling, ion channels, mechano-transduction, neuroeffector pathways, and cell-to-cell communication, all of which shape muscle excitability and contribute to disease processes.
Utilizing cutting-edge techniques, such as calcium imaging through spinning disk confocal microscopy and optogenetic approaches, as well as molecular biology and electrophysiological methods, we have made significant advancements in unraveling novel aspects of GI physiology. For instance, our pioneering work involves reporting calcium dynamics and its regulation in gut pacemakers “interstitial cells of Cajal (ICC)” and fibroblast cells at subcellular resolution using optogenetic sensors. Moreover, we have developed and applied innovative machine-learning imaging analysis techniques to ensure accuracy in defining calcium release sites and cell-to-cell interactions, avoiding any selection bias.
By leveraging these discoveries, our ultimate goal is to gain a deeper understanding of GI and bladder diseases, including overactive bladder, incontinence, irritable bowel syndrome, gastroparesis, and constipation. Through our research, we aim to provide valuable insights that can pave the way for more effective treatments and interventions in the future.
Subject expertise
- Gastroenterology
- Calcium signaling
- Gut pacemaker control
- Mechanisms of smooth muscle excitability
- Enteric nervous system
- Calcium image analysis
Education
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Diploma in Biomedical-Industrial Studies with Distinction. University of Ulster, 1999
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B.Sc. (Hons.) in Biomedical Sciences. University of Ulster, 2000
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Ph.D. Cellular and Molecular Physiology and Pharmacology. University of Nevada, Reno, 2008