Olympus-Based Spinning Disk Confocal and TIRF Microscope
- Olympus IX83 microscope
- 4 objectives
- 20x, NA 0.45, LUC PlanFl N (Air)
- 40x, NA 0.55, SLCPlanFl (Air)
- 60x, NA 1.49, Apo N (Oil)
- 100x, NA 1.50, UPlanApo (Oil)
- Automated XY stage for multi-position acquisition
- TrueFocus Zero drift automatic focus
- Andor Integrated Laser Engine (ILE) and Borealis with 405-, 488-, 538-, and 640-nm solid-state lasers and integrated acousto-optic tunable filter (AOTF)
- The spinning disc confocal side consists of a Yokogawa CSU-21 Nipkow spinning-disk confocal scanner unit and an iXon X3 cooled EMCCD camera.
- The TIRF side contains a 2-line motorized Olympus IX3 CellTIRF Illuminator and an iXon Ultra cooled EMCCD camera.
- Seamless switching between confocal and TIRF imaging modalities
- Oko-Touch stage-top incubator provides temperature and CO2 regulation for long-term imaging of live cells
- CellSens Dimension software running on a Windows 10-based PC