The HSTRI Core manages a custom-built 3i Lattice LightSheet Microscope that is capable of 3D live cell imaging.
The Lattice LightSheet is a system designed to push the spatial and temporal resolution limits in live cell imaging.
Cylindrical lenses stretch and collimate the beam to form a sheet projected onto a spatial light modulator (SLM).
The SLM generates an optical lattice of Bessel beams.
An annular mask acts as a zero-order filter, removing artifacts and lengthening the sheet.
Lightsheet thickness is 0.4 µm at 50 µm length.
Galvos dither the sheet in X and sweep the sheet in Z.
25x/1.1NA 28x/0.71NA water immersion detection objectives
Piezo x,y translation stage and piezo imaging objective control.
Images are acquired using a high-speed high-resolution 2Kx2K Hamamatsu ORCA-Flash4.0 V3 sCMOS camera.
Samples are excited with 405, 488, 560, and 642 nm lasers.
Image acquisition and analysis are performed by 3i SlideBook software (v. 6) running under Windows 10 on a powerful desktop computer comprised of Dual 16-Core Xeon Gold 2.9GHz processors, 128GB RAM, 8GB NVIDIA Quadro RTX4000 workstation graphics card, 512GB OS SSD, 8TB Fast Acquisition Drive, and 20TB additional storage.
The system is also capable of Structured Illumination Microscopy (SIM), which allows breaking of the diffraction limit for single molecule imaging and photomanipulation.
