Summary
Ruben K. Dagda, Ph.D. received his doctoral training at the University of Iowa and his postdoctoral training at the University of Pittsburgh School of Medicine. He is currently investigating the molecular mechanisms that lead to mitochondrial dysfunction and oxidative stress in cell culture, tissue and animal models of Parkinson's disease.
He has authored in multiple research manuscripts and review articles in the areas of toxicology, toxinology, mitochondrial function, and neurobiology. At the University of Nevada, Reno School of Medicine, he is committed to the training and education of undergraduate, graduate students and postdocs in his lab. His main research goals are to elucidate the prosurvival signaling pathways that regulate mitochondrial function, transport and turn-over in neurons and how aging and neurodegenerative diseases negatively impact these processes. The end goal is to develop novel small molecular drugs that can reverse neurodegeneration and elevate mitochondrial function in age-related neurodegenerative diseases.
Research
Persistent dephosphorylation by mitochondrial localized protein phosphatases (protein phosphatase 2A) accelerates neurodegeneration, fragments mitochondria, and impairs mitochondrial function. On the other hand, mitochondrial serine/threonine kinases PTEN induced kinase 1 (PINK1) and PKA confer neuroprotection and regulate overlapping mitochondrial functions. Neurons rely on functionally efficient mitochondria to power critical neuronal functions. Given that impaired mitochondrial turnover and dysfunction underlie the etiology of many neurodegenerative diseases, understanding how reversible phosphorylation at the mitochondria regulates mitochondrial function, and turnover will lay the basic groundwork for developing future "mitoprotective" therapies for reversing mitochondrial dysfunction and neurodegeneration. PINK1 and mitochondrial PKA converge at the outer mitochondrial membrane (OMM) to regulate overlapping mitochondrial functions. At the postsynaptic compartment, both ser/thr kinases remodel dendritic arbors in developing neurons and regulate mitochondrial transport.
We are examining how mitochondrial PKA and PINK1 interact at the mitochondria and at the neurites to regulate mitochondrial function, dendritic morphology and survival.
A second are of interest it to determine how mitochondrial turnover (mitophagy) is regulated by reversible phosphorylation. We will examine whether mitochondrial ser/thr kinases and phosphatases regulate mitochondrial turnover by phosphorylating and destabilizing protein-protein interactions of specific components of the "mitophagosome" complex at the OMM by applying proteomics and biochemical approaches in neurons. A final goal this project is to synthesize functionalized nanoreagents that can activate prosurvival signaling pathways at the mitochondria as mitochondrial therapy for reversing mitochondrial pathology induced by neurodegenerative diseases and by normal brain aging.
Recent Conferences
2014
- Das Banerjee,T., Dagda, R., Dagda, R.K. 2014. PTEN-induced kinase 1 (PINK1) and Protein Kinase A protects Against mitochondrial dysfunction and dendritic loss in a toxin model of PD. American Society for Investigative Pathology: Experimental Biology Conference, San Diego.
- Rice, M., Dagda, R.K., Strack, S. 2014. Role of protein phosphatase 2A in modulating autophagy and mitophagy. American Society for Biochemistry and Molecular Biology: Experimental Biology Conference, San Diego, CA.
2013
- Dagda, R.K., Gusdon, A., and Chu, CT. 2013. Role of Distinct Subcellular Pools of PINK1 on Mitochondrial Function, Neuronal Morphology and Survival. 2013 Society for Free Radical Biology and Medicine Conference, San Antonio, TX.
- Dagda, R. K. 2013. Regulation of Mitophagy by Serine/Theronine Kinases and Cardiolipin at the Outer Mitochondrial Membrane: Implications for Mitochondrial Diseases. Gordon Research Conference on Cellular and Molecular Mechanisms of Toxicity, Proctor Academy, Andover NH.
Education
- Ph.D., University of Iowa, Pharmacology, 2006
- M.S. University of Texas at El Paso, Biology, 2001
- B.S. University of Texas at El Paso, Microbiology, 1998