Leica Stellaris 8 STED Super-Resolution Microscope

Lab workstation equipped with a computer and the Leica Stellaris 8 STED Super-Resolution Microscope
  • Leica DMi8 inverted fluorescence microscope equipped with Adaptive Focus Control and Closed Loop Focus (20 nm re-positioning accuracy)
  • LED light engine for eyepiece visualization with filter cubes (DAPI, CFP, GFP, Rhod LP) and a scanning XY Stage.
  • Assay Editor for multiwell plate screening
  • Super Z high-speed / high precision Z galvo stage for XYZ, XYZT, XZY, and XZYT high-speed imaging.
  • 4 objectives
    • 40x, NA 1.30, Plan APO (Oil) – 0.17mm FWD
    • 86x, NA 1.20, Plan APO motorized correction collar STED (Water) – 0.3mm FWD
    • 93x, NA 1.30, Plan APO STED motorized correction collar (Glycerol) – 0.3mm FWD
    • 100x, NA 1.40 Plan APO STED (Oil) - 0.13mm FWD
  • Lasers
    • White Light Supercontinuum Laser Extreme (440 nm-790 nm)
    • 405 nm Diode - 50 mW CW
  • High performance 8 channel crystal Based Acousto optical beam splitter with 10 µs switch time. · A Tandem Scanner FOV + 8K Resonant Point Scanner with prism-based spectral imaging (extended red range 410 nm - 850 nm).
  • Light detection is accomplished via Power HyD Detectors:
    • 2 Spectral HyD S (high QE/PDU, highest dynamic range, high light level OK, photon counting, spectral, FLIM)
    • 2 Spectral HyD X (high QE/PDU, high dynamic range, photon counting, spectral, ideal for FLIM)
    • 1 Spectral HyD R (red optimized high QE/PDE 720 nm - 850 nm, high dynamic range, photon counting, spectral, ideal for FLIM)
    • PMT based brightfield detector.
  • Lightning Mode (spectral, multi-channel super-resolution imaging: 120 nm XY & ~200 nm Z resolution at 1.4 NA; works with all detectors and all objectives)
  • 3D Tau STED nanoscopy with 592 nm / 660 nm / 775 nm depletion lasers (86x, 93x & 100x STED WHITE objectives (sub 50 nm XY resolution; sub 130 nm Z resolution; multi-channel, multi- color standard, gated or Tau STED)
  • Spectral Fluorescence Lifetime Imaging (FLIM; FALCON Fast FLIM, FLIM FRET, Phasor Analysis; Tau sense average photon arrival time with gating, contrast, and separation).
  • The system is controlled by LAS X Software running on a high-performance HP Z6G4 computer workstation comprising 2 HD monitors, 192 GB RAM / 6 TB HDD storage, Nvidia Quadro RTX 6000 graphics with 24 GB memory, 4608 CUDA cores, and 2 Thunderbolt ports (TB3).
  • OKO Labs opaque microscope enclosure environmental control with active mixing CO2, Humidity, and Heat.