Measles Virus RNA Real-time (TaqMan®) RT-PCR

Data	Value
Method	Real-Time RT-PCR, Real Time Reverse Transcription Polymerase Chain Reaction (q-PCR) Amplification of Measles Virus (SH) RNA.
CPT Code	87798
Test Scope	N/A
Collection Instructions	Detection of measles RNA and measles virus isolation are most successful when samples are collected on the first day of rash through the 3 days following onset of rash. However, virus may still be recovered by cell culture through day 10 following rash onset. Detection of measles RNA by RT-PCR may be successful as late as 10-14 days post rash onset. Throat, Nasopharyngeal (preferred), or nasal swabs. Synthetic swabs are recommended. Swab should be transferred to 1-3ml of viral transport medium (do not allow to dry out). Indicate specimen source on requisition.
Other Acceptable Specimens	N/A
Required Specimen Volume	N/A
Specimen Container	Viral Transport Media.
Specimen Identification	Label specimen with patient's first and last name, DOB, and date/time of collection.
Shipping Requirements	Specimen should be kept at 4°C (refrigerated) and transported to the laboratory within 24 hours of collection. Ship with cold-pack. If longer storage is required, the specimen should be frozen at -70 °C or on dry ice.
Sample Rejection Criteria	Improper specimen transport Mislabeled specimen Unlabeled specimen Wood shaft swabs
Turn-around-time	48-72 Hours (M-F)